[초청강연] Human assembloids to study the basic principle of human diseases

Human assembloids to study the basic principle of human diseases

Kunyoo Shin, Ph.D
Current technology for human organoids is limited in that it fails to provide essential patterning
cues and adequate signals to induce the complexity of the mature human tissues. Recently,
we created “human assembloids” derived from two vital tissues in our body, brains and
epithelial tissues, and established novel conceptual framework to overcome the major
limitations of current organoid technology. These assembloids exhibit the characteristics of
mature tissues in the context of cell compositions at the single-cell transcriptome level, and
recapitulate the in vivo tissue dynamics and complex cellular interplays during the various
physiological and pathological conditions. By developing patient specific mix-and-match
human assembloids combined with integrated genetic and epigenetic analysis, our recent
works further provided new mechanical insights into the development of various human
diseases from cancers to neurological diseases, which is controlled by complex interaction
between multiple cell types at distinct developmental and pathological stages in human
tissues. Our study also proposed an innovative preclinical model system to study a range of
human diseases, whose understanding of pathogenesis requires an organoid system that is
capable of representing mature characteristics of functional human tissues with multiple cell
types.