최희정

Choi, Hee-Jung

Professor
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최희정

Professor

Choi, Hee-Jung

최희정
Research
Biochemistry Biophysics Molecular Biology

Structural and biochemical studies by using molecular biology and biochemical techniques as well as X-ray crystallography.

 


Our target proteins are,

 

(1) GPCRs (G-protein coupled receptors)

: beta2-adrenergic receptor, Frizzled receptors

Here is the crystal structure of agonist bound active beta2-adrenergic receptor.


Choi et al., (2011) Nature, 469, 175-180.

 


(2) proteins involved in crosstalk between cell-cell adhesion system and Hippo signaling pathway

: alpha-catenin, beta-catenin, Merlin, YAP, LATS & MST kinases

Here is the crystal structure of vinculin alpha-catenin peptide complex.


Choi et al., (2012) Proc. Natl. Acad. Sci. USA, 109, 8576-8581.
Education/Career
Education
  • - 1993.3 – 1997.8 Ph.D., Dept. of Chemistry (X-ray Crystallography), Seoul National University
  • - 1991.3 – 1993.2 M.S., Dept. of Chemistry (Biochemistry), Seoul National University
  • - 1987.3 – 1991.2 B.S., Dept. of Chemistry, Seoul National University
Career
  • - 2021.9 – present Professor, Seoul National University
  • - 2016.9 – 2021.8 Associate Professor, Seoul National University
  • - 2012.9 – 2016.8 Assistant Professor, Seoul National University
  • - 2003.11 – 2012.8 Research Associate, Stanford University
  • - 2000.4 – 2003.10 Postdoctoral fellow, Stanford University
  • - 1997.11 – 2000.3 Postdoctoral fellow, Korea Research Institute of Bioscience and Biotechnology
Publications
  1. Choi H.-J., Pokutta S., Cadwell, G.W., Bobkov A.A., Bankston L.A., Liddington R.C., and Weis W.I. (2012) aE-catenin is an autoinhibited molecule that coactivates vinculin. Proc. Natl. Acad. Sci. USA, 109, 8576-8581.
  2. Ahn V.E., Chu M.L.-H., Choi H.-J., Tran D., Abo A., and Weis W.I. (2011) Structural Basis of Wnt Signaling Inhibition by Dickkopf Binding to LRP5/6. Dev. Cell, 21, 862-73.
  3. Choi H.-J. and Weis W.I. (2011) Crystal Struture of a Rigid Four-Spectrin-Repeat Fragment of the Human Desmoplakin. J. Mol. Biol., 409, 800-812.
  4. Choi H.-J., Rasmussen S.G. F., Fung J.J., Pardon E., Casarosa P., Chae P.S., Devree B.T., Rosenbaum D.M., Thian F.S., Kobilka T.S., Schnapp A., Konetzki I., Sunahara R.K., Gellman S.H., Pautsch A., Steyaert J., Weis W.I., Kobilka B.K. (2011) Structure of a nanobody-stabilized active state of (2) adrenoceptor. Nature, 469, 175-180.
  5. Rosenbaum D.M., Zhang C., Lyons J.A., Holl R., Aragao D., Arlow D.H., Rasmussen S.G., Choi H.-J., Devree B.T., Sunahara R.K., Chae P.S., Gellman S.H., Dror R.O., Shaw D.E., Weis W.I., Caffrey M., Gmeiner P., Kobilka B.K. (2011) Structure and function of an irreversible agonist-(2) adrenoceptor complex. Nature, 469, 236-240.
  6. Kwiatkowski A.V., Maiden S.L., Pokutta S., Choi H.-J., Benjamin J.M., Lynch A.M., Nelson W.J., Weis W.I., Hardin J. (2010) AlphaE-catenin regulates actin dynamics independently of cadherin mediated cell-cell adhesion. Proc Natl Acad Sci U S A., 107, 14591-6.
  7. Dimitrova Y.N., Li J., Lee Y.T., Rios-Esteves J., Friedman D.B., Choi H.-J., Weis W.I., Wang C.Y., Chazin W.J. (2010) Direct ubiquitination of beta-catenin by Siah-1 and regulation by the exchange factor TBL1. J. Biol. Chem., 285, 13507-16.
  8. Bokoch M.P., Zou Y., Rasmussen S.G., Liu C.W., Nygaard R., Rosenbaum D.M., Fung J.J., Choi H.-J., Thian F.S., Kobilka T.S., Puglisi J.D., Weis W.I., Pardo L., Prosser R.S., Mueller L., Kobilka B.K. (2010) Ligand-specific regulation of the extracellular surface of a G-protein-coupled receptor. Nature, 463, 108-12.
  9. Choi H.-J., Gross J.C., Pokutta S., Weis W.I. (2009) Interactions of plakoglobin and beta-catenin with desmosomal cadherins: basis of selective exclusion of alpha- and beta-catenin from desmosomes. J. Biol. Chem., 284, 31776-88.
  10. Rosenbaum, D.M., Cherezov, V., Hanson, M. A., Rasmussen, S. G. F., Thian, F. S., Kobilka, T. S., Choi, H.-J., Yao, X.-J., Weis, W. I., Stevens, R. C. and Kobilka, B. K. (2007) GPCR Engineering Yields High-Resolution Structural Insights into 2 Adrenergic Receptor Function. Science, 318, 1266-1273.
  11. Cherezov, V., Rosenbaum, D.M., Hanson, M. A., Rasmussen, S. G. F., Thian, F. S., Kobilka, T. S., Choi, H.-J., Kuhn, P., Weis, W. I., Kobilka, B. K. and Stevens, R. C. (2007) High-Resolution Crystal Structure of an Engineered Human 2-Adrenergic G Protein–Coupled Receptor. Science, 318, 1258-1265.
  12. Choi, H.-J., Rasmussen, S. G. F., Rosenbaum, D. M., Kobilka, T. S., Thian, F. S., Edwards, P. C., Burghammer, M., Ratnala, V. R. P., Sanishvili, R., Fischetti, R. F., Schertler, G. F. S., Weis, W. I. & Kobilka, B. K. (2007) Crystal structure of the human beta2 adrenergic G-protein-coupled receptor. Nature, 450, 383-388.
  13. Day, P. W., Rasmussen, S. G. F., Parnot, C., Fung, J. J., Masood, A., Kobilka, T. S., Yao, X.-J., Choi, H.-J., Weis, W. I., Rohrer, D. K. & Kobilka, B. K. (2007) A monoclonal antibody for G protein coupled receptor crystallography. Nature Methods, 4, 927-929.
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