윤태영

교수

윤태영 Yoon, Tae-Young

윤태영
연구분야
분자생물학 생물물리학 생화학

Revealing native folding pathways for membrane proteins: My research group aims to understand how membrane proteins form their tertiary or quaternary structures. To answer this complex question, we employ a diverse array of single-molecule biophysics and cell biology tools to observe membrane protein folding in vitro as well as in cellular conditions, in unprecedented details. Before delving into this question, we have gained our experiences on how to apply single-molecule techniques to various membrane-involving biological processes. We have reported the disassembly mechanism of the SNARE complex by a proteasome system (20S complex) that provides important insights into how AAA+ ATPases tightly couple their ATP hydrolysis with unfolding of protein substrates (JACS 2013, Science 2015 and Nat. Comm. 2021). Our efforts finally culminate in a single-molecule approach based on magnetic tweezers, which permits observation of detailed folding pathways of multi-pass membrane proteins with a resolution of a few amino acids (Nat. Comm. 2013, 2014, 2018, Nat. Chem. Biol. 2015, Science 2019, and Nat. Chem. Biol. 2022). Our lab hopes to reveal the folding pathways of important human proteins, which may in turn shed lights into their evolutionary history, ER membrane protein homeostasis, and facilitation of folding of these membrane proteins for industrial applications.

Development of Tools for Precision Medicine: My research group is also interested in development of new single-molecule imaging tools. We recently developed single-molecule version of co-immunoprecipitation (co-IP) analysis (Nat. Comm. 2013, Nat. Prot. 2013). The co-IP analysis has been used as the gold standard of determining protein-protein interactions (PPIs) in the modern molecular biology for several decades. By adopting single-molecule fluorescence microscopy as the detection method (instead of SDS PAGE-gel and western blotting), we have improved the sensitivity and time-resolution of the co-IP analysis by five orders of magnitude, respectively. With the extreme sensitivity and quantitativeness of the developed tool, we demonstrate development of PPI biomarkers that allow precision prediction of drug responses of individual tumors, in particular, in cancer types without actionable genomic mutations. This suggests a path toward “personalized diagnosis of cancers at the PPI level”, which would expand the concept and scope of the targeted cancer therapy (Nat. Biomed. Eng. 2018, eLife 2020).

 
학력/경력
학력
  • - 2000. 3 – 2004. 8 서울대학교 전기공학 박사
  • - 1998. 3 – 2000. 2 서울대학교 전기공학 석사
  • - 1994. 3 – 1998. 2 서울대학교 전기전자제어공학 학사
경력
  • - 2021.06 - 과학기술정보통신부 한국연구재단 리더연구사업 연구단장
  • - 2020.03 - 서울대학교 생명과학부 교수
  • - 2017.03 – 2020.02 서울대학교 생명과학부 부교수
  • - 2016. 03 – 2017.02 연세대학교 Yonsei-IBS 과학원
  • - 2014. 02 – 2016.02 한국과학기술원 물리학과 영년직 부교수
  • - 2011. 04 – 2020.02 한국연구재단 미래창조과학부 창의적연구진흥사업 연구단장
  • - 2010. 09 – 2014.02 한국과학기술원 물리학과 부교수
  • - 2007. 10 – 2010.08 한국과학기술원 물리학과 조교수
  • - 2006. 7 – 2007. 10 어바나-샴페인 소재 일리노이 주립대학 하워드 휴즈 의학 연구소 박사후연구원
  • - 2005. 7 – 2006. 7 어바나-샴페인 소재 일리노이 주립대학 물리학과 박사후연구원
  • - 2004. 9 – 2005. 6 서울대학교 반도체 공동 연구소 박사후연구원
주요논문
  1. Hyun-Kyu Choi, Hyunook Kang, Chanwoo Lee, Hyun Gyu Kim, Ben P. Phillips, Soohyung Park, Charlotte Tumescheit, Sang Ah Kim, Hansol Lee, Soung-Hun Roh, Heedeok Hong, Martin Steinegger, Wonpil Im, Elizabeth A. Miller, Hee-Jung Choi and Tae-Young Yoon (2022), Evolutionary balance between foldability and functionality of a glucose transporter, Nature Chemical Biology (18), 713-723.
  2. Hyun-Kyu Choi, Hyun Gyu Kim, Min Ju Shon, and Tae-Young Yoon (2022), High-resolution single-molecule magnetic tweezers, Annual Review of Biochemistry (91), 33-59.
  3. Byoungsan Choi, Minkwon Cha, Gee Sung Eun, Dae Hee Lee, Seul Lee, Muhammad Ehsan, Pil Seok Chae, Won Do Heo, Yongkeun Park and Tae-Young Yoon (2020). Single-molecule functional anatomy of endogenous HER2-HER3 heterodimers, eLife(9), e53934.
  4. H.-K. Choi, D. Min, H. Kang, M. J. Shon, S.-H. Rah, H. Jeong, H.-J. Choi, J. U. Bowie & T.-Y. Yoon (2019) Watching helical membrane proteins fold reveals a common N- to C-terminal folding pathway. Science
  5. M. J. Shon, H. Kim & T.-Y. Yoon (2018) Focused clamping of a single neuronal SNARE complex by complexin under high mechanical tension. Nature Communications